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A semen analysis evaluates certain characteristics of a male’s semen and the sperm contained in the semen. It may be done while investigating a couple’s infertility or after a vasectomy to verify that the procedure was successful. It is also used in stud farming, dog feeding and farm animal breeding.
A small, uncalibrated drop from a well mixed undiluted specimen is placed in the center of the Chamber by means of a simple rod and immediately covered. A microscopic objective of x20 is required.
Semen is the turbid, whitish substance that is released from the penis during ejaculation. Sperm are the cells in semen with a head and a tail that enables them to travel to the egg. A sperm contains one copy of each chromosome and fuses with the female’s egg, resulting in fertilization.
Tests that may be done during a semen analysis include:
Non-motile sperm are counted within an area of nine or sixteen squares in the center of the grid. Moving sperms are then counted, and graded if desired. The procedure is repeated in several areas. Percentage of motility and its quality are then calculated. .
A part of the original specimen is transferred to another test tube for immobilization by placing the tube in hot water (50o-60o): a cup with 2/3 boiling water and 1/3 tap water is suggested. A drop of the immobilized specimen is then placed in the Chamber and counting initiated: sperm heads within a ten square area are counted in the same manner as blood cells are counted in a hemocytomer, their number represents their concentration in millions per ml. In cases of oligospermic semen, sperms in the entire grid area are to be counted, representing their concentration in hundreds of thousands.[/vc_column_text][/vc_column][/vc_row][vc_row][vc_column][vc_message style=”square” message_box_color=”mulled_wine”]
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